Abstract: Lipid content and composition of different parts of fluted pumpkin seeds, were investigated. The percentage yield of oil extracts from the samples were (29.5-37.±0.3 ) and (0.5-25±0.1) for the seeds and seed coats, respectively. The following lipids were identified; phosholipids, glycolipids, sterols, fatty acids and neutralipids. These lipid classes were isolated and identified using different organic extract ants and thin-layer chromatography, respectively. The various RF values of the sample were matrix matched with the standard RF values of known lipids.