Abstract: The aim of this research was to investigate suitable detoxification method(s) for thevetia seed meal. This should lead to the production of a meal with significantly reduced cardiac glycoside content with minimal loss of nutrient. Acid hydrolysis of the defatted meal followed by alcoholic extraction of the freed aglycones with 80% aqueous alcohol gave a detoxified meal with 95% reduction in the toxic glycoside content (from 42.7 g kg-1 to 2.15 g kg-1) and a 3.9% increase in crude protein (from 42.79 to 44.45%) but with a 48% loss of meal (100 g defatted meal giving 52 g detoxified meal), while direct alcoholic extraction of the glycoside led to a 98% reduction in the glycoside content (from 42.7 g kg-1 to 0.83 g kg-1); a 25.3% increase in crude protein content (from 42.79 to 53.6%) and an 18% loss of material (with 100 g defatted meal giving 82 g detoxified meal). The results showed that alcoholic treatment is more effective for reducing the toxin content of thevetia meal with minimal loss of material and nutrient than acid hydrolysis.