Abstract: A Prime number is a natural number larger than one which cannot be expressed as the product of two smaller natural numbers. Prime numbers have been shown to have significant applications in nature as shown by the life cycle of cicadas of the genus Magicicada. Determining the exact distances between prime numbers is an important unsolved problem in mathematics. This study investigated the distances between prime numbers generated from nucleotides in a segment of Bacteriophage T4 genome. The digits of Euler’s number e and Pi π, two mathematical constants were found to be encoded spatially between the Viral DNA helical segments comprised of prime numbers.