Abstract: This research was conducted to know the effect of professional competence and organizational culture to the lecturer performance in University of PGRI Palembang. Population in this research was permanent lecturers in University of PGRI Palembang who already achieved lecturer certification and received lecturer’s professional allowance for 65 lecturers and this population also used as research sample (saturated sampling). The research type was quantitative research method using correlational study approach and data analysis using survey method with multiple linear regression. The research result showed that independent variable (professional competence and organizational culture) in this research able to give contribution of 36.3% to the lecturer performance. The result of hypothesis test stated that simultaneously, professional competence and organizational culture affected positively and significantly to the lecturer performance in University of PGRI Palembang while partially, professional competence and organizational culture affected positively and significantly to the lecturer performance in University of PGRI Palembang.