Abstract: The process of isolating humic substances, especially, humic acid (extraction, fractionation and purification) is time consuming, laborious and expensive. The objective of this study was to determine the minimum period required to fractionate humic acid from paddy husk compost. The rice straw compost was produced by mixing 80% of shredded rice straw, 10% of goat manure slurry, 5% of chicken feed and 5% of molasses. During the extraction process, 10 g of compost was placed in the centrifuge bottle, added with 100 mL of 0.5 M NaOH. The samples were equilibrated at room temperature for 24 h. After that, the mixture was centrifuged at 10, 000 G for 15 min. During the fractionation process, the pH of the solution was adjusted to 1.0 by using 6 M hydrochloric acid. Then the humic acid was allowed to equilibrate for 1, 3, 5, 7, 9, 12 and 24 h, respectively and the suspension was centrifuged. Purification was done subsequently to purify the humic acid.The yield of humic acid fractionated under 1 h was 0.26 g and was not statistically different from 3, 5, 7, 9, 12 and 24 h. This indicates the fractionation period of 1 h also had the same time taken as 3, 5, 7, 9, 12 and 24 h for the exchange sites of humic acid of compost to be saturated with H+ions after acidification. Phenolic, carboxylic and total acidity were determined and falls within the range reported by literatures. The optimum fractionation period of humic acid from paddy husk compost was 1 h as the yield of humic acid under different fractionation periods were not significantly different. Therefore, by using 1 h of fractionation period it will absolutely save the consumption of chemicals, electricity and labor costs.