Abstract: Bone Marrow (BM) derived Mesenchymal Stem Cells (MSCs) are pluripotent cells which can replicate as undifferentiated cells, have potential to differentiate into endothelial, osteogenic, adipogenic and other lineages under determined culture conditions and are considered as a valuable source for the in vivo investigation of tumor angiogenesis, cell and gene based therapy. The aim of this study was to optimize in vitro expansion of MSCs derived Endothelial Progenitor Cells (EPCs) in a rat model. MSCs were isolated from bone marrow by a combination of gradient density centrifugation and adherence properties. in vitro cultures of MSCs were established using the 6 different commercially available Growth Media (GM) including DMEM, DMEM+VEGF, Endopan, MV2 and Panserin, and their potential to differentiate into osteogenic, adipogenic and endothelial cells was shown. By using Endopan MSCs exhibit the highest proliferation rate and the uppermost capacity to EPCs differentiation in comparison with other exploiting GM. Endopan is favorable medium for EPCs preparation. It may be very helpful in the different applications of EPCs in small-animal cell based diagnostics and therapeutic models.