Abstract: In this study, the morphological and karyological peculiarities of six (4 , 2 ) specimens of Pipistrellus pipistrellus collected from Diyarbakir province were examined. The external and cranial measurements and weights of the specimens were taken and also the field notes were recorded. The diploid chromosome number of the specimens was 2n = 42, fundamental chromosome number was NF = 52 and autosomal chromosome number was NFa = 48. The X chromosome was large submetacentric and Y chromosome was small acrocentric. The external and cranial measurements and karyological and morphological peculiarities were compared with previously published accounts. The results revealed that the subspecies of P. p. pipistrellus distributed in this region.