Abstract: The influence of nutritional factors on parameters of the hemostatic system has been investigated in various studies. The main purpose of this study was to determine the effect of sunflower oil intake on coagulating factors, serum cholesterol and triglgycerides. In 2006 with randomized clinical trial study, 21 healthy young men were selected for study (18-28 years old ). After take of 24 recall hour, objectives consumes 30 g sunflower oil for 28 days and blood sample was taken for test before and after 2nd and 4th week. The results indicated that serum cholesterol decreases and Ptt increases significantly at the end of second and fourth week (p< 0.05 ), but triglycerides and other coagulating factors was not different (p>0.05). This study indicat that high unsaturated fat diet intake may be decrease serum cholesterol and increase ptt in healthy individuals.