Abstract: This study was performed to determine the pattern of antimicrobial resistance of Shigella sp. in patients with acute diarrhea. The study included all patients with acute diarrhea who were visited in a teaching hospital during 14 months period (2004- 2005). The isolates were confirmed by biochemical reaction and specific antisera. Antibiotic susceptibility test was determined by agar diffusion method. Of the 273 stool samples, 130(47.6%) yielded Shigella sp. Shigella flexneri caused 94(72.3%) of the total cases of shigellosis followed by S. dysenteriae 17 (13.1%), S. boydii 15 (11.5%) and S. sonnei 4 (3.1%). All 130 isolates showed resistance at least to one of eleven antibiotics and 123/130 (94.6%) were resistant to two or more antimicrobial agents. The high number of multi-drug resistance isolates and decreased susceptibility to main antibiotics for treatment of Shigella sp. warrant need to continuous monitoring of antimicrobial susceptibilities through a surveillance system for effective therapy and control measures.