Abstract: Bacterial lipopolysaccharides are the major outer surface membrane components present in almost all Gram-negative bacteria and act as extremely strong stimulators of innate or natural immunity in diverse eukaryotic species ranging from insects to humans. The DNA sequence of the O-antigen biosynthesis cluster of a putative probiotic and pathogenic strain, Vibrio alginolyticus has been determined. Here, we report the sequence of the LPS biosynthesis genes, wzm, wzt and wbil and the analysis of the genes using Biology Workbench 3.2. From the study, it shows that the sequences of LPS genes in V. alginolyticus are highly homologous to the LPS genes in Vibrio cholerae isolates with more 80% homology. However, several variants of the wbil sequence have been found in the V. alginolyticus isolates compared to the other genes, wzm and wzt.