Abstract: The isolation of Bacillus strains with antifungal activity is important to carry out tests to protect economically important crops. We determined the in vitro activity of 13 native strains of Bacillus sp. isolated from soil against Macrophomina pasheolina using a dual assay in nutrient agar. All the strains showed inhibition of radial growth of M. phaseolina from 31-80%, with strains LUM B01 and B04 in a 80.8 and 75.9%, respectively with the highest antifungal activity. The crude extract of the strain of chitinases LUMB0 04 inhibited the growth of M. phaseolina by 30%. The strains of chitinolytic Bacillus sp. isolated from soils showed antifungal activity and have the potential to be used in biological control of M. phaseolina.