Abstract: The objective of this study was to determine the protein requirement for the maintenance of male Thai native cattle fed with Pangola hay as roughage. Eight cattle with body weight of 104±10.3 kg were arranged in double 4x4 Latin square design. Cattle were fed with dietary Crude Protein (CP) levels of 5.0, 7.0, 9.0 and 11.0% CP with similar amounts of metabolizable energy. Crude protein digestibility increased (p<0.05) with increasing levels of CP. Moreover, Nitrogen (N) intake, N absorption and N retention increased (p<0.05) with increasing levels of CP. Prediction equation N retention (g kg-1 BW0.75) with relation to N intake (g kg-1 BW0.75) was N retention = - 0.616 + 0.930 N intake (R2 = 0.993, SE = 0.056, p<0.001, n = 8). From these equations, it can be explained that the CP requirement for the maintenance of male Thai native cattle is 0.662 gN kg-1 BW0.75 or 4.14 gCP kg-1 BW0.75.