Abstract: Tooth enamel is the hardest and most highly mineralized substance of the body and with dentin, cementum and dental pulp is one of the four major tissues which make up the tooth in vertebrates. The unique microstructure of enamel resides fossilized tracks of its growth process. These tracks represent the incremental growth of enamel. Forensic odontology is a vital and integral part of forensic science. Essential data could be derived by the study of the enamel structure and contribute in dental identification, age estimation procedures and archaeology, anthropology and forensic researches. Further advances in image analysis and computer technology would enhance the knowledge and improve the accuracy of methods used in Forensic odontology field.