Abstract: The thermosensitive hydrogel for cartilage tissue engineering was developed by blending Chitosan (C) and Starch (S) with β-Glycerol Phosphate (GP). This C/S/GP hydrogel showed its liquid state at room temperature and gel state at raised temperatures. Scanning Electron Microscope (SEM) images revealed interconnected pore structure having pore sizes in the range of 30-120 μm. The material gradually degraded by 44.7 and 57.9% within 8 weeks in Phosphate Buffer Saline (PBS) and PBS containing 1 mg mL-1 lysozyme, respectively. The biocompatibility of C/S/GP hydrogel was initially evaluated by cultivating with Adipose-Derived Stem Cells (ADSCs). The SEM observation showed superior structure of the C/S/GP hydrogel with cells compared to hydrogel without cells. Cell adhesion and cell proliferation were also demonstrated by using SEM image and XTT assay, respectively. This study shows that the C/S/GP hydrogel is potentially applicable as an injectable scaffold for further study in cartilage tissue regeneration from ADSCs.