Abstract: Different methods have been proposed in the course of time of age estimation via human biological characteristics. One of them is the measurement of dentine translucency. The amount of transparent dentine that is deposited through time is strongly related with the age of the person. Some controversy on the accuracy of the method has been stated, thus it is evident by numerous studies that the sclerotic dentin that is formed during aging due to altering rates of mineralization has successfully lead in age estimation of experimental subjects. Finally, the results of the past studies are coherent with the current literature, proving that age estimation via root dentine translucency is possible in clinical basis.