Abstract: Lactic Acid Bacteria (LAB) were isolated from different fermented fruits and vegetables and screened for antifungal activity against chilli phytopathogen Colletotrichum gloeosporioides. Seven LAB showed antifungal activity as observed in the dual overlay assay method. These LAB grew between 10 and 45°C, pH 4.4 and 9.6 (except 1 isolate), 6.5 and 18% NaCl. Fungi infected seeds from four species of chilli were evaluated for germination rate with and without LAB treatments. LAB isolated C5 showed good fungal inhibition and allowed good seed germination and was identified as Lactobacillus plantarum using API CH50L kit. The results indicate that C5 has the potential to be used as a biological control for treatment of chilli seeds against C. gloeosporioides to replace the use of chemical fungicides.