Abstract: Inflammation is a non-specific reaction of body tissues against harmful stimulus, injury or infection to protect the body and promote the recovery process. Myeloperoxidase a pro-inflammatory enzyme released by activated neutrophils and macrophages is frequently used as a marker of polymorphonuclears accumulation in different tissue samples. Haruan (Channa striatus) is a tropical, snakehead, freshwater, air breathing and carnivorous fish belongs to family channadae which is indigenous to Malaysia. The current study was undertaken to identify the properties of a new haruan based cream as a natural product on myeloperoxidase enzyme as a marker of polymorphonuclears accumulation. In the present study, anti-inflammatory activity of haruan (Channa striatus) based cream (at doses 1, 5 and 10%) on the ear thickness and myeloperoxidase activity (as two acute inflammation indexes) were studied using croton oil induced ear edema method and compared with effects of hydrocortisone 1% cream as reference drug. Results showed that all percentages of haruan based cream in a same potent with reference treatment significantly inhibited edema at 4 and 24 h after croton oil application (p<0.05). Besides, myeloperoxidase assay results shown that topical application of these three different dosages of haruan based cream also block migration of polymorphonuclear leukocytes to the dermis as effective as hydrocortisone cream 1% and in a dose dependent manner (p<0.05). Evidences obtained from the present study, on the one side, support the previous results that had shown the anti-inflammatory effects of haruan extracts in other animal models and on the other side, introduce a new topical biomedical product with a potency as great as hydrocortisone in inhibition of inflammation. It is suggested that topical anti-inflammatory effects of haruan based cream is contributed to its bioactive substances content such as linoleic acid, stearic acid, oleic acid and N-arachidonylplycine.