Abstract: Mycotoxins are harmful substances produced by fungi in various foods and are estimated to affect, as much as 25% of the world’s crop each year. Most of these mytocoxins belong to the 3 genera of fungi: Aspergillus, Penicillium and Fusarium. Gilan and Mazandaran provinces are located in the North of Iran with favorite conditions for Aspergillus growth. In present study, researchers will study the production of Deoxynivalenol toxin in cell extract of Aspergillus species isolated indigenous in North of Iran. After recognition of the species using ELISA, researchers quantitatively analysis Deoxynivalenol produced by available species. Finally, the greatest DON mean concentration in the biomass based on the farms isolates was related to mountainous region with maximum amount and followed by related to the forestrial and interstitial regions, respectively.