Abstract: The study specified the problem of microbial flora microbiological studies improvement for a human skin in the context of an effective law enforcement and the fight against the most socially significant crimes. The high scientific and practical potential of the resident microflora is conditioned by the nature and the composition of skin bacteria which together with sweat and fat substance remain on a man’s clothes and environmental things. This process is more complicated but also more important for the purposes of law enforcement, rather than a person’s identity determination according to the bacterial colonies on the ridges of papillary patterns. As the researcher summary, the key issues in forensic microbiology have a positive solution and practical importance for the investigation activity.