Abstract: Medicinal plants have limited side effects and lower cost than pharmaceutical drugs. Thus, the present study was conducted to compare the antifungal effect of Eucalyptus globules L. and Teucrium polium L. extracts with Nystatin in-vitro. In this experimental study, the hydro-alcoholic extract (50, 100 and 250 mg L–1) was obtained through percolation method for Eucalyptus globulus L. and Teucrium polium L. separately. Candida albicans was cultured in Mueller Hinton Agar and a disk containing each concentration of the plants, a disk containing Nystatin as a positive control and a disk containing distilled water as negative control were placed in each culture plate. After 48 h, the mean diameter of the growth inhibition zone for each extract was compared with the mean diameter of the growth inhibition zone for Nystatin. Data were analyzed by SPSS Software (Version 21). The mean diameter of the growth inhibition zone around the disks containing eucalyptus extract in all concentrations was lower than Nystatin (p<0.001). The Teucrium polium L. extract did not indicate any effect on the development of Candida albicans in all concentrations. The hydro-alcoholic extract of Eucalyptus globules L. showed moderate antifungal effect against Candida albicans compared with Nystatin.