Abstract: The antibacterial activity of ethanol and ether extracts of 30 medicinal plants used against poultry diseases in Masaka district of Uganda was evaluated. The extracts were tested against gram positive (Staphylococcus aureus, Streptococcus faecalis) and gram negative bacteria (Escherichia coli, Salmonella typhimurium) using the agar well diffusion assay. Both ethanol and ether extracts showed activity (100 and 97%, respectively) against at least one bacterium. In general, gram positive bacteria were more susceptible than gram negative bacterial species. Of the four bacteria species, Staphylococcus aureus was the most susceptible to both extracts. These results therefore suggest that these plants can be used to provide lead compounds which can be used in the discovery of new antibacterials.