Abstract: The single-dose pharmacokinetics of ibafloxacin was studied in clinically normal chickens after intravenous and intramuscular administration of 7.5 mg of ibafloxacin kg‾1 of body weight. Ibafloxacin concentrations were determined by Microbiological assay method. The distribution and elimination half-lives were 0.261 and 5.87 h, respectively. Steady-state volume of distribution and clearance of ibafloxacin after intravenous administration were 4.09 L kg‾1 and 0.519 L kg‾1 h‾1, respectively. Following intramuscular administration, ibafloxacin achieved maximum plasma concentration of 1.06 μg mL‾1 achieved after maximum time of 1.3 h post-injection. The systemic bioavailability after intramuscular injection was 79.6% and the extent of serum protein binding was 27.04%. Ibafloxacin could be useful in the treatment of systemic infections in chickens after specific assessment of susceptible microorganisms.