Abstract: The objectives of this study identified the organizational culture in universities, that seen from a organizational culture comparison of old and new organizational culture. In this research, researcher used several techniques which are: indept interview, observation and documentation. The findings show: first, the old organizational culture with democratic leadership proved can be well built, where the Islamic culture and the Syekh-Yusuf figure has become the spirit and strength for implementation of Tridharma colleges. Second, new organizational culture can be built less well with autocratic leadership. Organization culture can provide benefits in troubleshooting in organization.