Abstract: The purpose of this study, was to compare the virulence between the two local Sudanese strains of Pasteurella multocida B and E. The virulence experiment has been conducted in calves and rabbits. The calves and rabbits were divided into three groups; group (I) was infected with strain B, group (II) was infected with strain E and group (III) was determined as control. The finding of this study, showed that P. multocida local Sudanese vaccine strain B is more virulent than strain E.