Abstract: The present study was undertaken to determine the efficacy of 32 different antibiotics against 38 S. aureus isolates obtained from milk samples from cattle with subclinical mastitis. About 6 antibiotics, i.e., Azithromycin, Gentamycin, Chloramphenicol, Tobramycin, Netillin and Neomycin were found 100% effective followed by Methicillin and Linezolid (94.74%), Cotrimoxazole (92.11%), Nitrofurazone (92.10%), Enrofloxacin (89.47%), Bacitracin (84.21%) and Sparfloxacin, Amoxyclav, Cefaclor and Amoxycillin (81.58%). The resistance was 71.05% for Vancomycin and Polymyxin B, 50% for Oxytetracycline, 47.37% for Azlocillin and 36.84% for Novobiocin whereas Cefixime and Metronidazole were completely (100%) ineffective against all the isolates.