Authors : Fuying Zheng, Guozhen Lin, Changqing Qiu, Jizhang Zhou, Xiaoan Cao and Xiaowei Gong
Abstract: The infected blood was collected from dairy cattle showing similar Bovine Ephemera Fever (BEF) clinical signs in Taizhou area of Zhejiang province. The blood frozen-thawed 3 times was concentrated decuple by centrifugation, then the virus had 7 blind passages in suckling mice by an intracerebral inoculation route and 10 passages in BHK21 cells. The suckling mice died regularly 3-4 days after inoculation from the 3rd passage with 100% mortality rate. In BHK21 cells, the CPE occurred from the secondary passage and 80% cells approximately emerged pathological changes post-infection from the 4th passage. The virus isolated was proved to be Bovine Ephemera Fever Virus (BEFV) by RT-PCR, Transmission Electron Microscopy (TEM), Virus Neutralization (VN) test and IFA test. The virus isolated was designated JT02L. The homologies of amino acid sequence of the G gene compared with JB76H (Beijing strain), Taiwan strain and BB7721 (Australia’s strain) all were above 90%.
Fuying Zheng, Guozhen Lin, Changqing Qiu, Jizhang Zhou, Xiaoan Cao and Xiaowei Gong, 2009. Isolation and Characterization of a Field Strain of Bovine Ephemeral Fever Virus in China. Journal of Animal and Veterinary Advances, 8: 1478-1483.