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Journal of Animal and Veterinary Advances
Year: 2009 | Volume: 8 | Issue: 4 | Page No.: 779-781
Genotyping of Kappa-Casein Locus by PCR-RFLP in Brown Swiss Cattle Breed
Unsal Dogru and Memis Ozdemir
 
Abstract: A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) test was performed on DNA samples extracted from blood samples of Brown Swiss cattle to detect genotype frequency of the bovine kappa-casein (CNS3) locus. A 351 bp fragment of CSN3 was amplified and digested with Hinf I restriction enzymes. Samples were loaded on agarose jel (3%) and genotyped under UV light. Three genotypes were observed, frequencies were 19.35, 20.43 and 60.22% for AA, BB and AB, respectively. Two genetic variants CSN3 A and CNS3 B were identified and the allelic frequencies were estimated as 0.495 and 0.505, respectively. It was concluded that the population was in Hardy-Weinberg equilibrum.
 
How to cite this article:
Unsal Dogru and Memis Ozdemir , 2009. Genotyping of Kappa-Casein Locus by PCR-RFLP in Brown Swiss Cattle Breed. Journal of Animal and Veterinary Advances, 8: 779-781.
URL: http://medwelljournals.com/abstract/?doi=javaa.2009.779.781