Authors : Xiong Wang, Li Ma and Ze-Xiang Jin
Abstract: Fructus Hordei Germinatus (Poaceae, Hordeum) has been traditionally used for treatment of female galactorrhea or Hyperplasia of Mammary Gland (HMG) in China. The overall purpose of this study was to evaluate the Anti-Hyperplasia of Mammary Gland (HMG) effect of Fructus Hordei G Ethanol extract (FHG-E) in HMG rats. Forty virgin female Wistar rats were randomly divided into blank control, HMG Model, Rupisanjie Capsule (RPSJC, positive control) and FHG-E groups, 10 in each. Injection of estrogen and progestogen were given to prepare rat models of HMG, RPSJC and FHG-E were given at the same time. Changes of nipple heights were measured sex hormone levels (E2, P, PRL, FSH and LH) in serum were estimated uterus and ovory index were calculated. Pathologic changes of mammary gland in rats were also observed by microscope. FHG-E could decrease the increased nipple height and uterus index, reduce the numbers of mammary gland lobules and secretion in HMG rats. It could also decrease E2, PRL and FSH levels in serum and increase the serum levels of P and LH significantly compared with the model group. The histopathological observation revealed that FHG-E obviously alleviated the degree of HMG. The contents of total flavonoid total alkaloid and total phenolic contents in the extract were also determined. The results showed that the 50% ethanolic extract from FHE contained total flavonoids 3.95±0.06 mg Quercetin Equivalent (QE)/g extract total alkaloids 42.74±0.08 mg Hordenine Equivalent (HE)/g extract and total phenolics 52.46±0.12 mg Gallic Acid Equivalent (GAE)/g extract. These results suggest FHG-E has therapeutic effects on HMG rats induced by estrogen and progestogen.
Xiong Wang, Li Ma and Ze-Xiang Jin, 2013. Therapeutic Effect of Fructus Hordei Germinatus Ethanol Extract on Hyperplasia of Mammary Gland in Rats. Journal of Animal and Veterinary Advances, 12: 954-958.