Authors : Jianfeng Wang, Lichun Qian, Xiaoyan Weng and Jianyi Sun
Abstract: The objective of the study was to investigate the regulation of dietary Zinc (Zn) on transcription of key enzymes involved into hepatic lipid metabolism. Rats were fed a Zinc-Adequate diet (ZA, 46.39 mg Zn/kg basis diet), a Zinc-Deficient diet (ZD, 3.20 mg Zn/kg basis diet) and a Zinc-Overdose diet (ZO, 234.39 mg Zn/kg basis diet) for 5 weeks. Pair-Fed group (PF) rats were fed zinc-adequate diet with food intake of ZD group. Zinc concentrations of liver and femur and activity of Alkaline Phosphatase (ALP) in ZD group were significantly decreased compared with PF group whereas those in ZO group were markedly increased compared with ZA group. Content of oleic acid was markedly decreased in ZD but the contents of palmitic acid and oleic acid were significantly increased in ZO. Concentrations of hepatic Free Fatty Acid (FFA) and Triglyceride (TG) were markedly raised in ZD group whereas these in ZO group was reduced. Of 9753 genes analyzed by cDNA microarray, 13 genes related to hepatic lipid metabolism showed altered expression levels in livers of ZD and ZO rats, respectively with. The changes of four genes were confirmed by relative quantitative real-time RT-PCR. Compared with PF group, mRNA level of Acetyl-CoA Carboxylase (ACC) was significantly increased but Stearoyl-CoA Desaturase-1 (SCD-1) was reduced in ZD group. Relative to ZA group, mRNA levels of Fatty Acid Synthase (FAS) and ACC was obviously decreased in ZO group but these of Carnitine Palmiroyl Transferase-1 (CPT-1) and SCD-1 were significantly elevated. These results indicated that dietary zinc might affect hepatic fatty acid synthesis, β-oxidation and desaturation through regulation on FAS, ACC, CPT-1 and SCD-1 at the transcriptional level.
Jianfeng Wang, Lichun Qian, Xiaoyan Weng and Jianyi Sun, 2018. Transcriptome Analysis Identified the Regulation of Dietary Zinc on Hepatic Lipid Metabolism in Rats. Journal of Animal and Veterinary Advances, 17: 61-65.