Authors : Hanaa Mohamed Fadel, M.M Maather El-Lamie and Nahla Hamed Sallam
Abstract: To study the epidemiologic status of zoonotic parasitic diseases in some fish and shellfish species and fishermen, a total of 120 freshly caught fish; 100 cat fish (Clarias gariepinus) collected from Nile tributaries in Ismailia city, 20 keeled mullet (Liza Carinata), 400 alive marine shellfish; 100 shrimps (Penaeus japonicus), 100 gastropods (Thais carnifera) and 200 bivalves (Paphia andulata) in addition to 15 fecal samples were collected from fishermen at the shores of lake Temsah in Ismailia city, Egypt during (fall) 2017 and (Spring) 2018. The parasitological study revealed that the eggs of Fishborne Zoonotic Trematodes (FZTs) and Cryptosporidium spp. oocysts were detected in 26.7 and 20% of the examined fishermen stool samples, respectively. The prevalence of Encysted Metacercarial infection (EMC) of FZTs and cryptosporidiosis among the examined fin fish and shellfish species were (74 and 24%) in cat fish, (5 and 30%) in keeled mullet, (50 and 28 %) in shrimp, (20 and 30%) in gastropod and (60 and 20%) in bivalve. The main clinical picture of EMC affected fishes was slimness, haemorrhages all over the body with respiratory distress. Affected Penaeus japonicus were aggregated at the aerator sites while Thais carnifera and Paphia andulata showed no specific clinical picture. Cryptosporidiosis affected fishes showed emaciation, ascites and haemorrages along the stomach and intestine. Experimental infection in pigeons and rats, revealed Brachylaema migrans from pigeons fed on Penaeus japonicus and (Mesostephanus appendiculatus, Mesostephanus burmanicus, Procervum calderoni, Stictodora tanayensis, Apophallus donicus, Pygidiopsis genata, Maritrema orensense, Sphaeridiotrema globules, Prohemistomum vivax and Haplorchis pumilio) from albino rats fed on Clarias gariepinus. Some FZTs could not be identified by conventional morphologic typing, so, PCR targeting ITS2 (internal transcribed spacer) region was performed followed by bidirectional sequencing which revealed the following worms: Braunia cordiformis, Cyathocotyle prussica and Holostephanus dubinini.
Hanaa Mohamed Fadel, M.M Maather El-Lamie and Nahla Hamed Sallam, 2019. Surveillance of Parasitic Diseases of Zoonotic Importance in Fishermen, Some Fish and Shellfish Species. Journal of Animal and Veterinary Advances, 18: 175-186.