Authors : Isty Adhitya Purwasena and Yuichi Sugai
Abstract: Nutrition injection in Microbial Enhanced Oil Recovery (MEOR) process might stimulate not only desirable microorganisms but also the undesirable ones. Therefore, studying the properties of bacterial community in reservoir prior to field test is a critical step in MEOR process. In this study, bacterial communities from high thermic and low salinity reservoirs located in Japan, China and Indonesia were characterized by 16S rDNA sequence analysis. Bacterial genomic DNAs were extracted from produced water samples and their sequences were evaluated by genetic fingerprinting based approach. PCR-DGGE analyses showed multiple bands in all produced water samples which indicated high bacterial diversities. Sequences identified in this study were mainly related to only five phyla, i.e., Bacteroidetes, Thermotogae, Defferibacteres, Firmicutes and Proteobacteria, suggesting that phylotype richness is low in oil reservoirs. One sequence of Bacteriodetes-affiliated bacteria which showed 99% similarity with Bacteriodales bacterium 5bM was retrieved from DGGE results of all oilfields. This result showed that, Bacteriodetes can grow well in Indonesia, Japan and China oilfield, making it the best bacterial candidate for MEOR field test. Results showed in this study revealed that finger printing is a considerable technique for microbial screening prior to MEOR field test application.
Isty Adhitya Purwasena and Yuichi Sugai, 2018. The Analysis of Bacterial Diversity in High Thermic-Low Salinity Oil Reservoirs Prior to Microbial Enhanced Oil Recovery Field Test. Journal of Engineering and Applied Sciences, 13: 4104-4112.