Authors : Mochammad Taha Ma`ruf, Putu Sulistiawati Dewi, Ria Koesoemawati and Dewa Made Wedagama
Abstract: The objective of the study is to determine the efficacy of noni (Morinda citrifolia) extract gel to increase the number of macrophage and angiogenesis in mandibles socket after tooth extraction. In vivo experimental study consist of 32 guinea pigs divided into 4 unpaired groups, first group (control) treated with CMC-Na 2% gel, the rest (treatment groups) treated, respectively with noni extract gel of 10, 25 and 40%. Animals tooth extraction was performed, followed by gel application twice a day (morning and afternoon) for 1 min in accordance to the group. Guinea pigs were decapitated on the 5th day, post-extraction’s socket and the surrounding bone being removed, fixed and a series of histological examination was conducted. The amount of macrophage cells and angiogenesis were observed and calculated using binocular microscope. Analytic result shows a significant difference in the amount of macrophage and angiogenesis among the treatment group (p<0.05). Noni extract gel with 10% concentration was capable to increase the number of macrophage and angiogenesis compared to the concentration of 25, 40% and the control group. It can be concluded that noni extract gel 10% concentration can accelerate the healing process after tooth extraction of guinea pigs, indicated by an increased number of macrophage and angiogenesis. Flavonoid as an active compound found in noni could increase the number of macrophage as an immuno-stimulant by the activation of T-lymphocytes. Saponin accelerates angiogenesis resulting from changes in balance of activator and plasminogen inhibitor which affects the stimulation of angiogenesis.
Mochammad Taha Ma`ruf, Putu Sulistiawati Dewi, Ria Koesoemawati and Dewa Made Wedagama, 2019. An Analysis of Noni (Morinda citrifolia) Extract Gel to Increase the Number of Macrophage and Angiogenesis in Mandibles Socket After Tooth Extraction of Guinea Pigs. Journal of Engineering and Applied Sciences, 14: 9306-9309.