Abstract: Studies on the effect of time of pollination on seed set and viability in short glume sorghum was conducted on the Teaching a nd Research Farm of the Department of Crop Production and Horticulture Federal University of Technology, Yola. Two sorghum cultivars were used; The Treatments were 6 different timings on which controlled pollination was carried out, after emasculating the female flowers. These timings included 24, 27, 30, 48, 51 and 54 h. Data were collected on seven parameters namely; number of seed set per time, weight of 30 F₁ seeds, percent germination, vigor score, days to 50% flowering, days to 95% maturity and yield per plant to further establish seed viability. The data were subjected to analysis of variance based on Randomized Complete Block Design. Results showed that pollination conducted within 27-48 h out-performed others conducted at other timings; suggesting therefore that 27-48 h as the right time to perform crosses on sorghum in Yola environment.