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Research Journal of Biological Sciences
Year: 2009 | Volume: 4 | Issue: 9 | Page No.: 1032-1036
Synthesis and Antibacterial Activity of Silver/Montmorillonite Nanocomposites
Mansor Bin Ahmad , Kamyar Shameli , Majid Darroudi , Wan Md Zin Wan Yunus , Nor Azowa Ibrahim , Azizah Abdul Hamid and Mohsen Zargar
Abstract: Silver nanoparticles (Ag-NPs) were successfully synthesized into the interlayer space of Montmorillonite (MMT) by chemical reduction method. AgNO3 and NaBH4 were used as a silver precursor and reducing agent, respectively. The properties of Ag/MMT nanocomposites were studied as a function of the AgNO3 concentration. The UV-vis spectra of synthesized Ag-NPs showed that the intensity of the maximum wavelength of the plasmon peaks increased with increasing AgNO3 concentration. The crystalline structure of the Ag-NPs and basal spacing of MMT and Ag/MMT were also studied by Powder X-Ray Diffraction (PXRD). The antibacterial activity of Ag-NPs was investigated against gram-negative bacteria (Escherichia coli, Escherichia coli O157:H7 and K. pneumonia) and gram-positive bacterium (Staphylococcus aureus) by disk diffusion method using Muller-Hinton Agar (MHA) at different sizes of Ag-NPs. The smaller Ag-NPs were found to have significantly higher antibacterial activity. These results showed that Ag-NPs can be used as effective growth inhibitors in different biological systems, making them applicable to medical applications such as in surgical devices.
How to cite this article:
Mansor Bin Ahmad, Kamyar Shameli, Majid Darroudi, Wan Md Zin Wan Yunus, Nor Azowa Ibrahim, Azizah Abdul Hamid and Mohsen Zargar, 2009. Synthesis and Antibacterial Activity of Silver/Montmorillonite Nanocomposites. Research Journal of Biological Sciences, 4: 1032-1036.