Abstract: Measurement of corneal thickness is an important and necessary factor that plays an important role in diagnostic testing and treatment. Ultrasonic pachymetry which is currently the clinical gold standard method. Pentacam is a new imaging system for anterior eye segment study. The aim of this study was to compare central corneal thickness with ultrasound and pentacam method in patients with refractive errors. In a prospective study that performed in Ophthalmology Department of International Branch, Shiraz University on patients with refractive error, central corneal thickness measurement using ultrasound parchymeter and pentacam in patient with refractive errors referred to the Mehr Ophthalmology Clinic Center in the Tabriz in 2012 evaluated. In this study, researchers evaluated Central Corneal Thickness (CCT) of 105 eyes (only right eye) of 105 patients with myopic astigmatism. About 45 of patients were male and 60 of them were female. Mean age of male patients was 29.53±7.88 and in female patients was 28.80±7.05 year (p = 0.617). Mean of Central Corneal Thickness (CCT) measured by ultrasonic pachymetry was 511.73±36.48 micrometer and mean of Central Corneal Thickness (CCT) measured by pentacam 521.11± 35.24 micrometer. Mean of Central Corneal Thickness (CCT) measured by ultrasonic pachymetry was significantly lower than mean of Central Corneal Thickness (CCT) measured by pentacam (p<0.001). Mean difference of 2 studied methods in measurement of Central Corneal Thickness (CCT) was 9.38±6.42 micrometer so that in overall of patients, pentacam measured the Central Corneal Thickness (CCT) 9-10 micron greater than ultrasonic pachymetry. Significant direct liner correlation was found between Central Corneal Thickness (CCT) measured by ultrasonic pachymetry with Central Corneal Thickness (CCT) measured by pentacam (p<0.001).