Authors : Mahkameh Mirkarimi, Mahdieh Beheshti and Fatemeh Mahmoudi
Abstract: Adhesion of restorative materials to the tooth structure is significance in dentistry. The aim of this study was to evaluate the micro leakage of pit and fissure sealants following application of 1.23% Acidulate Phosphate Fluoride (APF). Thirty sound non carious human premolar teeth were randomly assigned in two groups (n = 20). Artificial fissure grooves were prepared on the buccal surface of each tooth. In test group 1.23% APF gel was applied for 4 min to thoroughly dried teeth and then teeth etched 15 sec using phosphoric acid gel. Bonding was applied to the fissures and polymerized with a light curing unit and the fissure sealant material (3M, USA) were placed on the fissures. In control group the procedure was the same as test group but with the omission of the fluoride application. All the teeth were thermo cycled 1000 times at 5-55oc with dwell time of 30 sec. Samples were immersed in 2% basic fuchsine solution for 24 h and the teeth were sectioned buccolingully for the middle of the fissure. Dye penetration was evaluated under stereomicroscope. In test group 20% of the specimens and 46.7% of those in the control group shows no dye penetration. The dye had penetrated to the base of the fissure in 26.7% of test group and 20% of controls. Comparing groups by Mann Whitney U-test revealed that the difference between groups was not significant (p<0.05). The results reveal that the use of topical fluoride treatment immediately prior to sealant placement does not increase micro leakage.
Mahkameh Mirkarimi, Mahdieh Beheshti and Fatemeh Mahmoudi, 2012. Micro Leakage Assessment of Pit and Fissure Sealant with Previous Fluoride Application: An in vitro Study. Research Journal of Medical Sciences, 6: 22-25.