Authors : Bijan Solimani, Boromand Chaharaein, Rabie Rahbar, Hassan Baneh and Vahid Hemmati
Abstract: Currently, microsatellites are the most popular markers in livestock genetic characterization studies. Microsatellite is extensively used for designing the genetic chain map, orienting quantity character loci and evaluating the genetic diversity. The aim of the present study was to detect genetic diversity of Sanjabi sheep breed inferred from microsatellite markers and their association with fecundity and body weight traits. Blood samples were randomly collected from 100 Sanjabi sheep, transported to the laboratory and stored at -4°C for further analysis. DNA was extracted using modified salting-out method and 11 microsatellite loci were amplified by specific primers pairs using Polymerase Chain Reaction (PCR). The PCR products were then separated by electrophoresis on 8% poly acryl-amid gel. Data was analyzed using GENEPOP and SAS software. Statistical analysis showed that all markers had significant effect on fecundity and some of the body weight traits (p<0.05).
Bijan Solimani, Boromand Chaharaein, Rabie Rahbar, Hassan Baneh and Vahid Hemmati, 2011. Genetic Diversity of Sanjabi Sheep Inferred from Microsatellite Markers and Their Association with Fecundity and Body Weight Traits. Research Journal of Animal Sciences, 5: 34-39.