Authors : R.T. Venkatachalapathy, Arjava Sharma, T.K. Bhattacharta and Soumi Sukla
Abstract: An investigation was carried out to identify single nucleotide polymorphism in DGAT locus of Indian cattle and buffalo breeds. DNA samples were collected from 368 animals belonging to four breeds of riverine buffalo (Bubalus bubalis) and three breeds of Indian cattle (Bos indicus). Polymerase chain reaction: Single Strand Conformational Polymorphism was carried out to detect single nucleotide polymorphism in 189, 201, 178, 257, 231 and 411 bp fragments from four breeds of buffalo. Two SSCP patterns, CC and CT were observed in the 411 bp fragment of buffalo and Indian cattle. Frequency of CC ranged from 0.41-0.59 in buffalo and 0.73-0.82 cattle breeds. The overall frequency of CC and CT genotypes were 0.51, 0.49 in buffalo and 0.76, 0.24 in cattle, respectively. Frequency of C allele was 0.74 and 0.88 in buffalo and cattle population, respectively. Sequencing of different alleles revealed a transition mutation C to T at 14th position of intron-8. PCR-SSCP of 189, 201, 178, 257 and 231 bp fragments showed monomorphic patterns in all the breeds of buffalo and cattle.
R.T. Venkatachalapathy, Arjava Sharma, T.K. Bhattacharta and Soumi Sukla, 2013. Single Nucleotide Polymorphism in DGAT1 Locus of Indian Cattle and Buffalo Breeds. Veterinary Research, 6: 68-71.