References

Al Dahouk, S., K. Nockler, H. C. Scholz, M. Pfeffer, H. Neubauer and H. Tomaso, 2007. Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp. Clin. Chem. Lab. Med., 45: 1464-1470.
PubMed  |  

Baily, G.G., J. B. Krahn, B.S. Drasar and N.G. Stoker, 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hyg., 95: 271-275.
PubMed  |  

Bogdanovich, T., M. Skurnik, P.S. Lubeck, P. Ahrens and J. Hoorfar, 2004. Validated 5 nuclease PCR assay for rapid identification of the genus Brucella. J. Clin. Microbiol., 42: 2261-2263.
CrossRef  |  

Born, G.L., J.R. Haynes and G.G. Burson, 1976. Blood culture technique based on centrifugation: Developmental phase. J. Clin. Microbiol., 3: 251-257.
Direct Link  |  

Bounaadja, L., D. Albert, B. Chenais, S. Henault, M.S. Zygmunt, S. Poliak and B. Garin-Bastuji, 2009. Real-time PCR for identification of Brucella spp.: A comparative study of IS711, bcsp31 and per target genes. Vet. Microbiol., 137: 156-164.
PubMed  |  

Bricker, B.J, 2002. PCR as a diagnostic tool for brucellosis. Vet. Microbiol., 90: 435-446.
PubMed  |  

Cloeckaert, A. and N. Vizcaino, 2002. DNA polymorphism and taxonomy of Brucella species. In: Brucella: Molecular and Cellular Biology, Lopez-Goni I. and I. Moriyon (Eds.), Horizon Bioscience, Norfolk, United Kingdom, pp: 1-24.

Corbel, M.J. and W.J. Brinley-Morgan, 1984. Genus Brucella Meyer and Shaw 1920, 173AL. Williams and Wilkins, Baltimore, MD.

Debeaumont, C., P.A. Falconnet and M. Maurin, 2005. Real-time PCR for detection of Brucella spp. DNA in human serum samples. Eur. J. Clin. Microbiol. Infect. Dis., 24: 842-845.
CrossRef  |  

Figge, R.M., M. Schubert, H. Brinkmann and R. Cerff, 1999. Glyceraldehyde-3-phosphate dehydrogenase gene diversity in eubacteria and eukaryotes: Evidence for intra- and inter-kindom gene transfer. Mol. Boil. Evol., 16: 429-440.
PubMed  |  

Gaviria-Ruiz, M.M. and N.M. Cardona-Castro, 1995. Evaluation and comparison of different blood culture techniques for bacteriological isolation of Salmonella typhi and Brucella abortus. J. Clin. Microbiol., 33: 868-871.
Direct Link  |  

Godfroid, J., C. Saegerman, V. Wellemans, K. Walravens and J.J. Letesson et al., 2002. How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet. Microbiol., 90: 461-477.
CrossRef  |  

Gopaul, K.K., M.S. Koylass, C.J. Smith and A.M. Whatmore, 2008. Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis. BMC Microbiol., 8: 86-99.

Herlich, M.B., R.F. Schell, M. Francisco and J.L. Le Frock, 1982. Rapid detection of simulated bacteremia by centrifugation and filtration. J. Clin. Microbiol., 16: 99-102.
Direct Link  |  

Leal-Klevezas, D.S., I.O. Martinez-Vazquez, A. Lopez-Merino and J.P. Martinez-Soriano, 1995. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals J. Clin. Microbiol., 33: 3087-3090.
Direct Link  |  

Lopez-Goni, I. and I. Moriyon, 2004. Brucella: Molecular and Cellular Biology. Horizon Bioscience, Norfolk, England, pp:1-428.

Mackay, I.M., K.E. Arden and A. Nitsche, 2002. Real-time PCR in virology. Nucleic Acids Res., 30: 1290-1305.
CrossRef  |  PubMed  |  Direct Link  |  

Navarro, E., J.C. Segura, M.J. Castano and J. Solera, 2006. Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis. Clin. Infect. Dis., 42: 1266-1273.
PubMed  |  

Newby, D.T., T.L. Hadfield and F.F. Roberto, 2003. Real-time PCR detection of Brucella abortus: A comparative study of SYBR green I, 59-exonuclease and hybridization probe assays. Applied Environ. Microbiol., 59: 4753-4759.

Nielsen, K., P. Smith, J. Widdison, D. Gall, L. Kelly, W. Kelly and P. Nicoletti, 2004. Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O157:H7. Vet. Microbiol., 100: 25-30.
CrossRef  |  Direct Link  |  

O'Leary, S., M. Sheahan and T. Sweeney, 2006. Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Res. Vet. Sci., 81: 170-176.
CrossRef  |  PubMed  |  

Office International des Epizooties OIE., 2008. Manual of standards for Diagnostic Tests and Vaccines. Office International des Epizooties O.I.E., Paris.

Queipo-Ortuno, M.I., J.D. Colmenero, J.M. Reguera, M.A. Garcia-Ordonez, M.E. Pachon, M. Gonzalez and P. Morata, 2005. Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples. Clin. Microbiol. Infect., 11: 713-718.
PubMed  |  

Romero, C., C. Gamazo, M. Pardo and I. Lopez-Goni, 1995. Specific detection of Brucella DNA by PCR. J. Clin. Microbiol., 33: 615-617.
PubMed  |  

Yagupsky, P., 1999. Detection of Brucellae in blood cultures. J. Clin. Microbiol., 37: 3437-3442.
Direct Link  |