Authors : Sadanand Fulzele , Raj Kumar Singh , Madhusudan Hosamani , Bimalendu Mondal and Mahendra Pal Yadav
Abstract: In the present study, we evaluated the diagnostic efficacy of attachment gene based PCR (A-PCR) and A-PCR-RFLP employing a number of capripoxvirus field samples received from different parts of the country during 2000-2005. The tests were compared with duplex PCR and A-PCR-RFLP methods. We also cloned and sequenced A-PCR products from three representative Goatpoxvirus (GPV) isolates and three representatives Sheeppox Virus (SPV) isolates. F-PCR products of two isolates of GPV were also cloned and sequenced. Sequence variation in the attachment and fusion gene fragments of SPV and GPV was also determined to confirm the differential amplification in the suspected clinical samples. Phylognetic analysis based on partial fusion gene sequence of capripoxviruses showed that Indian isolates are closely related to other GPV strains published earlier.
Sadanand Fulzele , Raj Kumar Singh , Madhusudan Hosamani , Bimalendu Mondal and Mahendra Pal Yadav , 2006. Evaluation of Duplex PCR and PCR-RFLP for Diagnosis of Sheep Pox and Goat Pox. International Journal of Tropical Medicine, 1: 66-70.