Authors : Mohammad Rafiqul Islam, Mahbub Jong Fateh Ali Taimur, Nivana Tanzim, Tazminaz Sultana, Yoon-Seok Roh and Bumseok Kim
Abstract: Fascioliasis, one of the most economically important helminth infestations of goats worldwide can be diagnosed using fecal examination and ELISA of serum samples. The aims of the present study were to compare ELISA and fecal examination for diagnosis of fascioliasis and to estimate the point prevalence of Fasciola gigantica in goats from two selected farms. Both serum and fecal samples (n = 100) were collected randomly from two goat farms. Antibodies against the f2 antigen of Fasciola were detected using ELISA. Fecal samples were examined using the modified McMaster technique. Among 100 goats, 38 serum samples showed the presence of antibody against fasciola and 21 of these had fasciola eggs. Overall, 27% goats were infested with fascioliasis with varying degrees of severity. The 28 sero-positive goats were retested for the presence of eggs and antibody 30 days after treatment with broad-spectrum anthelmintics. No eggs were found in the fecal samples but 24 goats remained sero-positive at varying degrees according to ELISA. The anthelmintics presumably destroyed the existing eggs and larvae but the antibody was still present and only detectable using ELISA. ELISA therefore offers a suitable technique for the rapid, specific and accurate detection of fascioliasis in goats.
Mohammad Rafiqul Islam, Mahbub Jong Fateh Ali Taimur, Nivana Tanzim, Tazminaz Sultana, Yoon-Seok Roh and Bumseok Kim, 2012. Sero-Diagnosis of Fasciola gigantica Infestations in Goats Using Elisa and its Comparison with Fecal Sample Analysis. Journal of Animal and Veterinary Advances, 11: 3806-3811.