Authors : S.E. Agarry , E. Betiku and B.O. Solomon
Abstract: Steady states of a continuous culture with an inhibitory substrate was used to estimate kinetic parameters under substrate limitation (chemo stat operation) Mixed cultures of an indigenous Pseudomonas fluorescence and Pseudomonas aeruginosa were grown in continuous culture on phenol as the sole source of carbon and energy at dilution rates of 0.010-0.20 h 1. Using different dilution rates several steady states were investigated and the specific phenol consumption rates were calculated. In addition, phenol degradation was investigated by increasing the dilution rate above the critical dilution rate (washout cultivation). The results showed that the phenol degradation by mixed culture of Pseudomonas fluorescence and Pseudomonas aeruginosa can be described by simple substrate inhibition kinetics under substrate limitation but cannot be described by simple substrate inhibition kinetics under washout cultivation. The phenol consumption rate (degradation rate) increased with increase in dilution rate. Fitting of the steady state data from continuous cultivation to various inhibition models resulted in the best fit for the Teissier kinetic inhibition model. The rsmax value of 0.322 mg/mg/hr obtained from the Teissier equation was comparable to the experimentally calculated rsmax value of 0.342 mg/mg/hr obtained under washout cultivation.
S.E. Agarry , E. Betiku and B.O. Solomon , 2007. Inhibition Kinetics of Phenol Degradation by Binary Mixed Culture from Continuous Culture and Wash-Out Data . Journal of Engineering and Applied Sciences, 2: 1020-1026.