Authors : A.G. Pogorelov, O.A. Suvorov, V.M. Bakhir, A.I. Panait, M.A. Pogorelova and G.R. Ivanisky
Abstract: Quantitative laser microtomography was employed to measure the single cell volume. NMRI mouse zygotes and 2-cell embryos were shown to exhibit the shrinkage at apoptosis induced with ROS (40 min., H2 O2 , 0.2 mM). Unlike early embryos, there was no influence of in vitro apoptotic circumstances on mature oocytes, direct precursor of early embryogenesis. Dulbecco’s solution prepared from electrochemically reduced bi-distilled water allows the low redox potential in both normal and apoptotic conditions. The data obtained permit us to suggest that ROS sub-milimolar concentrations likely act as the signaling impact. Cys-loo receptors containing thiol group may be considered as the target of hydrogen peroxide molecules.
A.G. Pogorelov, O.A. Suvorov, V.M. Bakhir, A.I. Panait, M.A. Pogorelova and G.R. Ivanisky, 2018. Indirect Response of Early Mouse Embryo to Oxidative Stress Evoked in Apoptosis Model. Journal of Engineering and Applied Sciences, 13: 4300-4304.