Authors : Onuma Chansatein and Parichat On-Ong-Arj
Abstract: Polyhydroxyalkanoates (PHAs) are biopolymers synthesized by microorganisms. Bacteria can accumulate PHAs in their cells and used as energy resource. In this study, PHAs-producing bacteria were isolated from rice by-products particularly Glutinous Broken Rice (GBR), non-glutinous Broken Rice (BRR), Rice Barn (RB) and Thai Rice Vermicelli (TRV). The samples were cultured on three cultural media including of Tryptic Soy Agar (TSA), Plate Count Agar (PCA) and Starch Agar (SA). The 356 of purified isolates were cultured on complex and minimal medium then screened for PHAs accumulation in their cells using Nile Red dyeing method. Detection of the intracellular granules was using florescent and transmission electron microscopy. Isolate BRR25-1 and RB36 were accumulated large areas of PHAs inclusion in their cells when cultured on SA and TSA, respectively. The isolates were sequenced based on 16S rRNA gene and submitted in GenBank with Accession No. MG745381 and MG745383. The potential isolates could be produced and accumulated PHAs in their cells when fermented in cheap carbon substrates from rice by-products.
Onuma Chansatein and Parichat On-Ong-Arj, 2019. PHAs Accumulation in Bacterial Cells Isolated from Rice By-Products. Journal of Engineering and Applied Sciences, 14: 3728-3731.