Authors : Ali Al-Dulaimi, Ahmed Nsaif Jasim, Zaid Abdulhadi Abed, Areeg Al-Hamadani, Mohammed Al-Gherairy, Rafid Al-Zuhairi Antimimi and Waleed Alewee
Abstract: In this research, we observed real-time production of single protein molecules in individual Escherichia coli cells using the fluorescence technique and the detection by localization method. They found that protein molecules are produced in burst, the number of protein in each burst varies and the burst exhibit particular temporal spreads (1). Although, they found that the burst happen arbitrarily, they decayed with time and the most important was that each gene expression burst results from one mRNA molecule. So, this research opened new avenues for many single molecule researches.
Ali Al-Dulaimi, Ahmed Nsaif Jasim, Zaid Abdulhadi Abed, Areeg Al-Hamadani, Mohammed Al-Gherairy, Rafid Al-Zuhairi Antimimi and Waleed Alewee, 2020. Gene Expression Probing in Live Cells, at Each Time One Protein Molecule. Journal of Engineering and Applied Sciences, 15: 2398-2404.