Authors : Hamad Ashraf, Nasreen Jamel Meo and Ikram-Ul-Haq
Abstract: The present study is concerned with the production of extracellular alpha amylase by Bacillus licheniformis GCUCM-30. The extracts of different brans such as soybean meal, rapeseed meal, canola meal, wheat bran, cottonseed meal, guar meal, sunflower meal and rice bran were supplemented to the fermentation medium and tested for alpha amylase production in 250 ml Erlenmeyer flask. Of all the extractants examined, 30% wheat bran and rice bran extract at the ratio of 7.5:2.5 was found to be the best for the synthesis of alpha amylase (574 U ml-1 min-1). The production of enzyme was reached maximum, 48h after inoculation.
Hamad Ashraf, Nasreen Jamel Meo and Ikram-Ul-Haq , 2003. Biosynthesis of Alpha Amylase by Bacillus licheniformis using Extracts of different Brans. Journal of Food Technology, 1: 173-177.