Authors : Roheena Abdullah, Hamad Ashraf and Ikram- ul- Haq
Abstract: The present study is concerned with the selection of carbon sources for the production of alpha amylase by Saccharomyces cerevisiae GCB-20. The lactose, glucose, maltose, xylose and sucrose as carbon sources were tested for the production of enzyme. Of all the sources tested, sucrose at the level of 0.5%was found to be best for the production of alpha amylase (530U/ml/min). The kinetic analysis indicated that the volumetric production of biomass and alpha amylase was significantly higher in the presence of sucrose (0.5%) added to the fermentation medium.
Roheena Abdullah, Hamad Ashraf and Ikram- ul- Haq , 2003. Optimization and Kinetic Analysis of Carbon Sources on the Production of Alpha Amylase by Saccharomyces cerevisiae. Journal of Food Technology, 1: 187-190.