Abstract: One of the contemporary problems faced in aquaculture is the provision of adequate nutritive and cheap feed to reduce the cost of fish production. In this study, the efficacy of Musa sapietum Inflorescence (MSI) as an ingredient in the diet of the juveniles of African mud catfish, Clarias gariepinus was evaluated over a 90 days growth period. About 3 experimental diets were formulated at 0 (control), 10 and 15% inclusion levels of MSI. The nutrient composition of MSI, growth performance and survival rate of the juvenile fish, feed intake and water quality parameters were determined using standard procedures. The results showed that MSI had a moisture content of 94.90% in the DM fat represented 2.08% and crude protein 1.85 %. MSI was a good source of potassium (24755 ppm of DM), calcium (9804 ppm in DM), sodium (8824 ppm in DM) and magnesium (3529 ppm in DM) but low in phosphorous (106 ppm in DM). There were no statistical differences found (p>0.05) in the specific growth rate, feed conversion and protein efficiency ratio of the juvenile fish fed with the banana blossom inclusions in comparison to the control. The 15% flower meal inclusion resulted in the highest fish survival rate (70%) compared to 63.3% (10% inclusion) and 60% (control). There were no significant differences (p>0.05) in the feed intake by the fish raised with 10 and 15% inclusions of MSI (34 and 33%, respectively) in comparison to the control that yielded 33%. There were no significant differences in the mean water pollution rates by nitrite accumulation (0.000, 0.275 and 0.042 mg dL^-1 in 15, 10 and 0% inclusions, respectively) and total dissolved solids (32, 35 and 33% in 15, 10 and 0% inclusions, respectively). The results obtained in this study indicated that M. sapietum flowers which are locally available in the rural areas and inexpensive have the potential to partially replace the expensive animal protein required in feed formulation and thus reduce cost in fish farming.