Authors : F. Alirachedi , F. Ferkous and Z. Djeghaba
Abstract: The aim of this reaearch is the extraction of an enzyme from dromedary liver and the study of its activity opposite to hydrolyse, esterification reactions and also the study of reactionnal medium effect. In first time, to determine the susceptible ester which will be hydrolysed by our enzymatic preparation, a number of substrates have been tried. We have also studied the influence of the acyl and alkoxyl parts of the ester on the hydrolysis reactions. Hence, we have noticed that the butyrates of butyl and ethyl are the most reactives among the group of esters used in our work. The obtained result is the same to that reached with the acetonic powder of the horse liver. In second time, we have studied the esterifications reactions on different substrates: carboxylic acids and alcohols and this, in the aim to surround the extract specifity. Finally, we note that the presence of proteinic impurities in the case of acetonic powders allows a better organic solvent stability, which in turn, allows a conservation of the enzyme in a given conformation and a suitable rate of hydration by preventing it from having a direct contact with the solvent. It is the reason why we have decided to combine the Acetonic Powder from the Liver of Dromedary (APLD) in different used solvents with each of the solvents that we have used.
F. Alirachedi , F. Ferkous and Z. Djeghaba , 2007. Hydrolysis and Esterification Reactions in Aqueous and Organic Medium: Study of the Selectivity Activity of an Acetonic Powder from the Dromedary Liver. Research Journal of Applied Sciences, 2: 136-140.