Abstract: The study was undertaken to differentiate hookworm species in Owerri and Port Harcourt during late rainy and early dry seasons, by the application of Harada-mori culture methods. Six hundred faecal. Samples (300 in each of the cities) were screened for positivity of the hookworm ova. Prevalence of hookworm infection was statistically higher in Owerri (23.3%) than Port Harcourt (13.3%, p< 0.005) and this was related to seasons of study. Sections of the cities with poor drainage and lower standard of hygiene (Diobu, Port Harcourt and Amakohia, Owerri) had higher prevalence rates (8%, 11.6%respectively) than those zones (Main towns) with improved standard of sanitation and adequate drainage facilities (prevalence rates: 1.3 and 3.3%, respectively). Thirty five of the fourty positive samples (87.5%) in Port Harcourt revealed the filariform larvae (second larval stage) of Necator americanus 25.5% were Ancylostoma duodenale: Similarly, 63 sixty three of the seventy positive samples (90%) obtained from Owerri were Necator americanus as against 10% which were A. duodenale. Both species were harvested from culture of fresh hookworm positive stool samples in Harada-mori apparatus for a period of ten days at room temperature (25-28�C). Predominance of N. americanus confirms with previous studies and appears to re-enforce the efficiency of Harada-mori culture method. The method was simple, cost effective and required minimum skills. Relative endemic status of the study areas were highlighted.